HT21
Cell Painting is a high-content imaging based technology developed at the BROAD institute that allows for the multidimensional characterization of cellular morphology (Bray MA et al. Nat Methods 2016). It is based on multiplexing six fluorescent dyes to capture eight cellular compartments or organelles enabling the generation of cell state-specific morphological profiles, providing highly valuable information for mechanistic interpretations (Nyffeler J et al., Toxicol Appl Pharmacol 389 2020). As such, morphological profiling of chemical entities combined with statistical analysis is gaining momentum in the field of pharmacology and toxicology as a methodology to identify MoA and toxicological effects (Warchal SJ et al., Bioorg Med Chem 28 2020).
Typically, morphological profiling is performed on adherent cells in 2D, in this project the student will be developing the method in cells grown in 3D.
Farmaceutisk vetenskap
Bioinformatik
Laborativ studie
N/A
Uppsala
Jordi Carreras-Puigvert
jordi.carreras.puigvert@farmbio.uu.se
30hp
1