ProjektportalA new method to quantify the mechanical stability of lipid-based drug nanocarriers using life-time fluorescence measurements.
Terrmin
HT23
Beskrivning
Lipid membranes lie at the core of cell membranes as well as of lipid-based nanoparticles intended for drug delivery. The mechanical properties of the lipid membrane are an important parameter to predict its stability and suitability for e.g. drug delivery applications. In this project, you will further develop a novel fluorescence-based method to study the rigidity of lipid membranes. The method is based on the quenching of a fluorescent dye encapsulated in liposomes (i.e. lipid bilayer vesicles) subjected to osmotic stress. In the initial stages of the project, you will refine already existing protocols to be able to relate the results from fluorescence measurements with the mechanical properties of lipid membranes with compositions relevant for drug delivery applications. In the final stages of the project, you will investigate how relevant therapeutic drugs and biomolecules affect the mechanical stability of lipid membranes.
During the project, you will use mainly fluorescence spectroscopy (both steady-state and life-time measurements) and will apply mathematical models to relate the obtained signals to the resistance of liposomes towards deformation. The fluorescence experiments can be complemented with Cryo-transmission electron microscopy (Cryo-TEM), dynamic light scattering (DLS).
Huvudområde
Farmaceutisk kemi
Ämne
Fysikalisk kemi
Typ
Laborativ studie
Företag
Uppsala Universitet
Ort/Plats
Uppsala
Handledarens namn
Victor Agmo Hernandez
Handledarens e-post
victor.agmo@ilk.uu.se
Institution
Institutionen för Läkemedelskemi
Program
Apotekarprogrammet
Kurs
Fördjupningsprojekt i farmaceutisk fysikalisk kemi 30 hp - 3FC290
Omfattning/hp
30hp
Hur många studenter kan antagas för detta projekt?
1