The influence of inflammatory bowel disease on the stability of orally delivered superparamagnetic iron oxide nanoparticles

Terrmin

HT23

Beskrivning

Purpose and specific aims
This project aims to test the stability, protein/enzyme corona formation, and aggregation behavior of superparamagnetic iron oxide nanoparticles (SPION) in media simulating the gastrointestinal tract (GIT) in healthy and inflammatory bowel disease (IBD) conditions.

The specific aims are:
i. To develop and characterize media simulating IBD conditions in each of the compartments of the gastrointestinal tract (stomach, small intestine, and colon) based on information available in the literature.

ii. To synthesize SPION by flame spray pyrolysis. Characterization of the nanoparticles will be performed by X-ray diffraction, transmission electron microscopy, Fourier Transform Infrared Spectroscopy, and Breuneur-Emmet-Teller surface area analysis.

iii. To test the aggregation behavior of SPION in the simulated media, in healthy and IBD-mimicking conditions. With both individual media incubation and sequential cascaded media. This would be achieved by measuring nanoparticle size with dynamic light scattering and nanoparticle tracking analysis.

iv. To characterize the protein corona formed on the surface of the SPION during their passage through the GIT in healthy and IBD-mimicking conditions. With both individual media incubation and sequential cascaded media.

v. Use a design-of-experiments approach to understand the media factors that influence the stability and aggregation behavior of the nanoparticles.

Huvudområde

Läkemedelsutveckling

Ämne

Läkemedelsformulering och Molekylär galenisk farmaci

Typ

Laborativ studie

Företag

Uppsala universitet

Ort/Plats

Uppsala

Handledarens namn

Alexandra Teleki

Handledarens e-post

alexandra.teleki@farmaci.uu.se

Institution

Institutionen för farmaci

Program

Masterprogram i läkemedelsutveckling

Kurs

Degree project in Drug Discovery and Development 45 c -3FK044

Omfattning/hp

45hp

Hur många studenter kan antagas för detta projekt?

1

© 2022 Uppsala universitet | Teknisk support