Gamma aminobutyric acid (GABA) modulates human T lymphocyte function

Terrmin

HT23

Beskrivning

Background
The gamma-aminobutyric acid (GABA) is the major inhibitory neurotransmitter in the nervous system playing an important role in the control of neuronal excitability. Interestingly, GABA also has immunomodulatory effect in the immune system and specific GABA signaling components are detected in a variety of immune cell types. The modulatory effect of the GABA system in immune cells is relevant to autoimmune and inflammatory diseases e.g., Type 1 Diabetes (T1D). Repurposing clinically approved GABAergic drugs may provide great opportunities to treat these diseases.
Aim
The aim of this project is to investigate the GABA signaling plasticity in human T lymphocytes under physiological and pathological conditions.
Material & Method
The regulatory rules about lab safety and working with human blood samples will be instructed. Peripheral blood mononuclear cells (PBMCs) and specific subtypes of T cells will be isolated from fresh blood samples or buffy coats of healthy donors and T1D patients when needed. A variety of experimental methods including quantitative PCR, Western blot, flow cytometry, immunocytochemistry, patch-clamp recording and Ca2+ imaging will be performed when appropriate. This project will further deepen our understanding of GABA-related signaling pathway networks in immune cells.

Huvudområde

Farmaceutisk vetenskap

Ämne

Fysiologi

Typ

Laborativ studie

Företag

Uppsala Universitet

Ort/Plats

BMC, Uppsala

Handledarens namn

Zhe jin

Handledarens e-post

Zhe.jin@mcb.uu.se

Institution

Institutionen för medicinsk cellbiologi

Program

Apotekarprogrammet

Kurs

Fördjupningsprojekt i fysiologi 30 hp - 3MC333

Omfattning/hp

30hp

Hur många studenter kan antagas för detta projekt?

1

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