Magnetic Biosensors for Non-invasive Diagnosis of Inflammatory Bowel Disease (IBD)

Terrmin

HT24

Beskrivning

The goal is to develop magnetic biosensors that can be administered orally to measure inflammatory biomarkers.


Background
Superparamagnetic iron oxide nanoparticles (SPION) have the potential to be used as orally delivered contrast agents for Magnetic Resonance Imaging. We will synthesize SPION by flame spray pyrolysis. The SPIONs will be functionalized with antibodies targeting luminal inflammatory bowel disease (IBD) biomarkers. Subsequently, the biosensor performance will be characterized in vitro. Ultimately, the goal is to develop magnetic biosensors that can be administered orally to non-invasively achieve a quantitative measurement of inflammatory biomarkers in vitro.

Methods
• Characterization of the synthetized SPION by XRD, FTIR, TGA, DLS, and ELS.
• Conjugation of the SPION to antibodies specific to IBD Biomarkers and characterization of its conjugation by SDS-PAGE, FTIR and TGA. First, a model protein will be conjugated to the nanoparticles. Once a protocol is established, the antibodies will be functionalized onto the SPION.
• The conjugated SPION will be incubated with relevant IBD biomarkers.
• The aggregation behavior of the SPION in the presence and absence of the biomarkers will be performed by DLS and ELS.
• If time permits, the nanoparticles will be tested under flow conditions in a cell-free biorelevant microfluidic system.

My tasks
• Characterize the SPION prior to any treatment.
• Conjugate the SPION to antibodies specific to IBD biomarkers.
• Characterize the conjugated SPION.
• Characterize the aggregation behavior of conjugated SPION upon the presence of IBD Biomarkers.

Expected results:
• Characterization of the aggregation behavior of conjugated SPION upon the presence of IBD biomarkers.

Huvudområde

Läkemedelsutveckling

Ämne

Läkemedelsformulering och Molekylär galenisk farmaci

Typ

Laborativ studie

Företag

Uppsala universitet

Ort/Plats

Uppsala

Handledarens namn

Alexandra Teleki

Handledarens e-post

alexandra.teleki@farmaci.uu.se

Institution

Institutionen för farmaci

Program

Masterprogram i läkemedelsutveckling

Kurs

Degree project in Drug Discovery and Development 45 c -3FK044

Omfattning/hp

45hp

Hur många studenter kan antagas för detta projekt?

1

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