ProjektportalFormation of electroactive supported lipid bilayers for the study of the membrane-partition behavior of ionic drugs.
Terrmin
VT22
Beskrivning
How do ionic drugs are transported across cell membranes? This question is still not convincingly answered in spite of its high relevance: most therapeutic drugs are charged at physiological pH.. How these drugs partition and diffuse through the cell membrane is still a matter of debate. A novel experimental technique developed in our laboratory can provide with insight about how ionic drugs partition into lipid membranes. The method is based on supported electroactive lipid bilayers. Upon oxidation or reduction of an electroactive probe embedded in the lipid bilayer, ionic drugs will be transferred into the membrane interior. The potential at which this process occurs can be related to the standard partition coefficient of the drug, independently of what other ions are present in solution.
In this project you will develop methods to produce electroactive supported lipid bilayers of various biologically relevant compositions on different supports (mainly gold and indium-tin oxide). These structures will then be used to characterize the membrane-partition behavior of reference ions and of relevant therapeutic ionic drugs. You will use electroanalytical techniques (mainly electrochemical impedance spectroscopy (EIS) and cyclic voltammetry) in combination with the quartz crystal microbalance with dissipation monitoring (QCM-D). Complementary techniques as dynamic light scattering (DLS) and Cryo-TEM may also be used.
Huvudområde
Farmaceutisk kemi
Ämne
Fysikalisk kemi
Typ
Laborativ studie
Företag
Institutionen för Läkemedelskemi, UU
Ort/Plats
Uppsala
Handledarens namn
Víctor Agmo Hernández
Handledarens e-post
victor.agmo@ilk.uu.se
Institution
Program
Kurs
Omfattning/hp
30hp
Hur många studenter kan antagas för detta projekt?
1